The new fund brought in both familiar names like Cortes Capital and S-Cubed Capital, plus fresh faces such as JIMCO.
It's already backing three startups: Reach Industries (robotics for labs), a University of Chicago spinout accelerating small-molecule discovery using computational chemistry, and a stealth company helping get scientific breakthroughs to market.
Originating from the Thiel Foundation's grant program, Breakout launched as a firm in 2016.
Breakout has steadily grown its funds, from $60 million in 2017 to this latest round.
Past wins include Surf Bio's acquisition by Halozyme in a deal valued at up to $400 million and portfolio companies landing major pharmaceutical deals.
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